Activators of Protein Kinase C Down-Regulate and Phosphorylate the T3/T-Cell Antigen Receptor Complex of Human T Lymphocytes
As judged by indirect immunofluorescence, phorbol 12, 13-dibutyrate and 1-oleoyl-2-acetylglycerol induced a rapid, concentration-dependent decrease of about 50% in the surface expression of the T3 antigen on human T lymphoblasts, and of T3 and the T-cell antigen receptor on HPB-ALL cells. Direct bin...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1985-12, Vol.82 (23), p.8158-8162 |
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| container_issue | 23 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Cantrell, Doreen A. Davies, Adelina A. Crumpton, Michael J. |
| description | As judged by indirect immunofluorescence, phorbol 12, 13-dibutyrate and 1-oleoyl-2-acetylglycerol induced a rapid, concentration-dependent decrease of about 50% in the surface expression of the T3 antigen on human T lymphoblasts, and of T3 and the T-cell antigen receptor on HPB-ALL cells. Direct binding experiments using125I-labeled antibody indicated that the reduction in T3 expression corresponded to a decrease in the number of antigen molecules rather than a change in their affinity. Biochemical analyses revealed that phorbol dibutyrate induced a rapid, prominent phosphorylation of the T3 Mr26,000 γ chain and to a lesser extent of the Mr21,000 δ chain. No phosphorylation of the T3 ε chain or of the α and β subunits of the T-cell antigen receptor was detected. The data suggest that protein kinase C induces a phosphorylation of the T3 γ and δ chains that may lead to the down-regulation of the T3/T-cell antigen receptor complex. |
| doi_str_mv | 10.1073/pnas.82.23.8158 |
| format | Article |
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Direct binding experiments using125I-labeled antibody indicated that the reduction in T3 expression corresponded to a decrease in the number of antigen molecules rather than a change in their affinity. Biochemical analyses revealed that phorbol dibutyrate induced a rapid, prominent phosphorylation of the T3 Mr26,000 γ chain and to a lesser extent of the Mr21,000 δ chain. No phosphorylation of the T3 ε chain or of the α and β subunits of the T-cell antigen receptor was detected. The data suggest that protein kinase C induces a phosphorylation of the T3 γ and δ chains that may lead to the down-regulation of the T3/T-cell antigen receptor complex.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.82.23.8158</identifier><identifier>PMID: 3934668</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Antibodies ; Antibodies, Monoclonal ; Antigens ; Antigens, Differentiation, T-Lymphocyte ; Antigens, Surface ; Cell Compartmentation - drug effects ; Cell lines ; Cell Membrane - metabolism ; Diglycerides - pharmacology ; Down regulation ; Enzyme Activation - drug effects ; Fluorescence ; Humans ; Macromolecular Substances ; Molecules ; Monoclonal antibodies ; Phorbol 12,13-Dibutyrate ; Phorbol Esters - pharmacology ; Phorbols ; Phosphorylation ; Protein Kinase C - physiology ; Receptors, Antigen, T-Cell - metabolism ; T lymphocytes ; T-Lymphocytes - metabolism</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1985-12, Vol.82 (23), p.8158-8162</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3768-1794fa881f5fe3b962464f5885cc1a1f357076f63773cc2945fbd7621d0238763</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/82/23.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/26540$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/26540$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3934668$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cantrell, Doreen A.</creatorcontrib><creatorcontrib>Davies, Adelina A.</creatorcontrib><creatorcontrib>Crumpton, Michael J.</creatorcontrib><title>Activators of Protein Kinase C Down-Regulate and Phosphorylate the T3/T-Cell Antigen Receptor Complex of Human T Lymphocytes</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>As judged by indirect immunofluorescence, phorbol 12, 13-dibutyrate and 1-oleoyl-2-acetylglycerol induced a rapid, concentration-dependent decrease of about 50% in the surface expression of the T3 antigen on human T lymphoblasts, and of T3 and the T-cell antigen receptor on HPB-ALL cells. Direct binding experiments using125I-labeled antibody indicated that the reduction in T3 expression corresponded to a decrease in the number of antigen molecules rather than a change in their affinity. Biochemical analyses revealed that phorbol dibutyrate induced a rapid, prominent phosphorylation of the T3 Mr26,000 γ chain and to a lesser extent of the Mr21,000 δ chain. No phosphorylation of the T3 ε chain or of the α and β subunits of the T-cell antigen receptor was detected. The data suggest that protein kinase C induces a phosphorylation of the T3 γ and δ chains that may lead to the down-regulation of the T3/T-cell antigen receptor complex.</description><subject>Antibodies</subject><subject>Antibodies, Monoclonal</subject><subject>Antigens</subject><subject>Antigens, Differentiation, T-Lymphocyte</subject><subject>Antigens, Surface</subject><subject>Cell Compartmentation - drug effects</subject><subject>Cell lines</subject><subject>Cell Membrane - metabolism</subject><subject>Diglycerides - pharmacology</subject><subject>Down regulation</subject><subject>Enzyme Activation - drug effects</subject><subject>Fluorescence</subject><subject>Humans</subject><subject>Macromolecular Substances</subject><subject>Molecules</subject><subject>Monoclonal antibodies</subject><subject>Phorbol 12,13-Dibutyrate</subject><subject>Phorbol Esters - pharmacology</subject><subject>Phorbols</subject><subject>Phosphorylation</subject><subject>Protein Kinase C - physiology</subject><subject>Receptors, Antigen, T-Cell - metabolism</subject><subject>T lymphocytes</subject><subject>T-Lymphocytes - metabolism</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUtrGzEURkVpSZ2060KhRbusxtZrJM2iCzN9JNTQENy1kGXJnjAjDSM5jaE_vpracZtNVoL7nfMJ7gXgHUZTjASd9V7HqSRTQqcSl_IFmGBU4YKzCr0EE4SIKCQj7DU4j_EOIVSVEp2BM1pRxrmcgN9zk5p7ncIQYXDwZgjJNh5-b3KvhTX8HH754tZudq1OFmq_hjfbEPttGPZ_J2lr4ZLOlkVt2xbOfWo21sNba2yfO2Edur61D2P11a7THi7hYt9l3eyTjW_AK6fbaN8e3wvw8-uXZX1VLH58u67ni8JQwWWBRcWclhK70lm6qjhhnLlSytIYrLGjpUCCO06FoMaQipVutRac4DUiVApOL8CnQ2-_W3V2baxPg25VPzSdHvYq6EY9TXyzVZtwr2iFGSfZnx18M4QYB-tOKkZqPIMaz6AkUYSq8QzZ-PD_jyf-uPecXx7zUXxM_xUot2vbZB9SJj8-S2bg_QG4i3njJ4LwkiH6B_LdpdA</recordid><startdate>19851201</startdate><enddate>19851201</enddate><creator>Cantrell, Doreen A.</creator><creator>Davies, Adelina A.</creator><creator>Crumpton, Michael J.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>19851201</creationdate><title>Activators of Protein Kinase C Down-Regulate and Phosphorylate the T3/T-Cell Antigen Receptor Complex of Human T Lymphocytes</title><author>Cantrell, Doreen A. ; Davies, Adelina A. ; Crumpton, Michael J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3768-1794fa881f5fe3b962464f5885cc1a1f357076f63773cc2945fbd7621d0238763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Antibodies</topic><topic>Antibodies, Monoclonal</topic><topic>Antigens</topic><topic>Antigens, Differentiation, T-Lymphocyte</topic><topic>Antigens, Surface</topic><topic>Cell Compartmentation - drug effects</topic><topic>Cell lines</topic><topic>Cell Membrane - metabolism</topic><topic>Diglycerides - pharmacology</topic><topic>Down regulation</topic><topic>Enzyme Activation - drug effects</topic><topic>Fluorescence</topic><topic>Humans</topic><topic>Macromolecular Substances</topic><topic>Molecules</topic><topic>Monoclonal antibodies</topic><topic>Phorbol 12,13-Dibutyrate</topic><topic>Phorbol Esters - pharmacology</topic><topic>Phorbols</topic><topic>Phosphorylation</topic><topic>Protein Kinase C - physiology</topic><topic>Receptors, Antigen, T-Cell - metabolism</topic><topic>T lymphocytes</topic><topic>T-Lymphocytes - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cantrell, Doreen A.</creatorcontrib><creatorcontrib>Davies, Adelina A.</creatorcontrib><creatorcontrib>Crumpton, Michael J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cantrell, Doreen A.</au><au>Davies, Adelina A.</au><au>Crumpton, Michael J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Activators of Protein Kinase C Down-Regulate and Phosphorylate the T3/T-Cell Antigen Receptor Complex of Human T Lymphocytes</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1985-12-01</date><risdate>1985</risdate><volume>82</volume><issue>23</issue><spage>8158</spage><epage>8162</epage><pages>8158-8162</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>As judged by indirect immunofluorescence, phorbol 12, 13-dibutyrate and 1-oleoyl-2-acetylglycerol induced a rapid, concentration-dependent decrease of about 50% in the surface expression of the T3 antigen on human T lymphoblasts, and of T3 and the T-cell antigen receptor on HPB-ALL cells. Direct binding experiments using125I-labeled antibody indicated that the reduction in T3 expression corresponded to a decrease in the number of antigen molecules rather than a change in their affinity. Biochemical analyses revealed that phorbol dibutyrate induced a rapid, prominent phosphorylation of the T3 Mr26,000 γ chain and to a lesser extent of the Mr21,000 δ chain. No phosphorylation of the T3 ε chain or of the α and β subunits of the T-cell antigen receptor was detected. The data suggest that protein kinase C induces a phosphorylation of the T3 γ and δ chains that may lead to the down-regulation of the T3/T-cell antigen receptor complex.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>3934668</pmid><doi>10.1073/pnas.82.23.8158</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1985-12, Vol.82 (23), p.8158-8162 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pubmed_primary_3934668 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Antibodies Antibodies, Monoclonal Antigens Antigens, Differentiation, T-Lymphocyte Antigens, Surface Cell Compartmentation - drug effects Cell lines Cell Membrane - metabolism Diglycerides - pharmacology Down regulation Enzyme Activation - drug effects Fluorescence Humans Macromolecular Substances Molecules Monoclonal antibodies Phorbol 12,13-Dibutyrate Phorbol Esters - pharmacology Phorbols Phosphorylation Protein Kinase C - physiology Receptors, Antigen, T-Cell - metabolism T lymphocytes T-Lymphocytes - metabolism |
| title | Activators of Protein Kinase C Down-Regulate and Phosphorylate the T3/T-Cell Antigen Receptor Complex of Human T Lymphocytes |
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