Activators of Protein Kinase C Down-Regulate and Phosphorylate the T3/T-Cell Antigen Receptor Complex of Human T Lymphocytes

As judged by indirect immunofluorescence, phorbol 12, 13-dibutyrate and 1-oleoyl-2-acetylglycerol induced a rapid, concentration-dependent decrease of about 50% in the surface expression of the T3 antigen on human T lymphoblasts, and of T3 and the T-cell antigen receptor on HPB-ALL cells. Direct binding experiments using125I-labeled antibody indicated that the reduction in T3 expression corresponded to a decrease in the number of antigen molecules rather than a change in their affinity. Biochemical analyses revealed that phorbol dibutyrate induced a rapid, prominent phosphorylation of the T3 Mr26,000 γ chain and to a lesser extent of the Mr21,000 δ chain. No phosphorylation of the T3 ε chain or of the α and β subunits of the T-cell antigen receptor was detected. The data suggest that protein kinase C induces a phosphorylation of the T3 γ and δ chains that may lead to the down-regulation of the T3/T-cell antigen receptor complex.