K 2P 2.1 channels modulate the pH- and mechanosensitivity of pancreatic stellate cells

Pancreatic stellate cells (PSCs) are central in the development of acute pancreatitis and tumor fibrosis in pancreatic ductal adenocarcinoma (PDAC). Fibrosis and a unique pH landscape represent characteristic properties of the PDAC microenvironment. Mechanosensitive ion channels are involved in the activation of PSCs. Among these channels, K 2.1 has not yet been studied in PSCs. K 2.1 channels are pH- and mechanosensitive. We confirmed K 2.1 expression in PSCs by RT-qPCR and immunofluorescence. PSCs from K 2.1 and K 2.1 mice were studied under conditions mimicking properties of the PDAC microenvironment (acidic extracellular pH (pH ), ambient pressure elevated by + 100 mmHg). Migration and the cell area were taken as surrogates for PSC activation and evaluated with live cell imaging. pH -dependent changes of the membrane potential of PSCs were investigated with DiBAC (3), a voltage-sensitive fluorescent dye. We observed a correlation between morphological activation and progressive hyperpolarization of the cells in response to changes in pH and pressure. The effect was in part dependent on the expression of K 2.1 channels because the membrane potential of K 2.1 PSCs was always more hyperpolarized than that of K 2.1 PSCs. Cell migration velocity of K 2.1 cells decreased upon pressure application when cells were kept in an acidic medium (pH 6.6). This was not the case in K 2.1 PSCs. Taken together, our study highlights the critical role of K 2.1 channels in the combined sensing of environmental pressure and pH by PSCs and in coordinating cellular morphology with membrane potential dynamics. Thus, K 2.1 channels are important mechano-sensors in murine PSCs.