Photoelectrochemical signal polarity transition mediated by quercetin for the detection of neuron-specific enolase

A photoelectrochemical (PEC) biosensor with a wide linear detection range was developed for the sensitive detection of neuron-specific enolase (NSE), which was achieved by applying a photocurrent polarity transition strategy mediated by quercetin. The coupling reaction between Cr( vi ) and quercetin drives the signal polarity from anodic to cathodic. When only quercetin is present in the test solution, photogenerated electrons are transferred to the electrode to generate anodic photocurrent. However, in the presence of the target, the signal probe released Cr( vi ), which interacted with quercetin, and the electron transfer direction was changed to achieve signal polarity conversion. Meanwhile, protoporphyrin-sensitized Bi:SrTiO 3 nanocubes were used as matrix photoactive materials to provide basic photocurrent. The doping of Bi element would adjust the bandgap of SrTiO 3 , and the organic-inorganic composite material exhibits good photostability and chemical stability that can maintain stable photoelectric properties over a long period of time. Such a novel signal polarity transition strategy greatly broadened the sensor detection to the range of 0.00007-170 ng mL −1 and obtained a relatively low detection limit (25 fg mL −1 ), which greatly improved the detection sensitivity and accuracy of the biosensor. A photoelectrochemical biosensor is developed for the sensitive detection of neuron-specific enolase, which achieved the photocurrent polarity conversion strategy mediated by quercetin.