Biological Detoxification of Aflatoxin B 1 by Enterococcus faecium HB2-2

Aflatoxin B (AFB ) contamination in food and feed is a global health and economic threat, necessitating the immediate development of effective strategies to mitigate its negative effects. This study focuses on the isolation and characterization of HB2-2 ( HB2-2) as a potent AFB -degrading microorganism, using morphological observation, biochemical profiling, and 16S rRNA sequence analysis. An incubation of HB2-2 at 32 °C for 96 h in a pH 10 nutrient broth (NB) medium resulted in a remarkable degradation rate of 90.0% for AFB . Furthermore, HB2-2 demonstrated 82.9% AFB degradation rate in the peanut meal, reducing AFB levels from 105.1 to 17.9 μg/kg. The AFB degradation ability of HB2-2 was found to be dependent on the fermentation supernatant. The products of AFB degradation by HB2-2 were analyzed by liquid chromatography-mass spectrometry (LC-MS), and a possible degradation mechanism was proposed based on the identified degradation products. Additionally, cytotoxicity assays revealed a significant reduction in the toxicity of the degradation products compared to the parent AFB . These findings highlight the potential of HB2-2 as a safe and effective method for mitigating AFB contamination in food and feed.