Assessment of possible biomedical applications of green synthesized TiO 2 NPs-an in-vitro approach

Biomedical applications for various types of nanoparticles are emerging on a daily basis. Hence this research was performed to evaluate the antifungal (Aspergillus sp., Alternaria sp., Trichophyton sp., Candida sp., and Penicillium sp.), cytotoxicity (MCF10A cell lines), and antioxidant (DPPH) potential of Coleus aromaticus mediated and pre-characterized TiO NPs were studied with respective standard methodology. Interestingly, the TiO NPs exhibited significant antifungal activity on pathogenic fungal strains like Alternaria sp., Aspergillus sp. (31 ± 1.4), Penicillium sp. (31 ± 1.9) Trichophyton sp. (27 ± 2.1), and Candida sp. (26 ± 2.3) at high concentration (250 μg mL ). However, the considerable levels of zone of inhibitions on fungal pathogens were recorded at 100 μg mL of TiO NPs as well as it was considerably greater than positive control. It also demonstrated dose based anti-inflammatory and antidiabetic activities. The plant-mediated TiO NPs demonstrated a maximum DPPH scavenging efficiency of 91% at a dosage of 250 μg mL , comparable to the positive control's 94%. Furthermore, TiO NPs at 100 μg mL concentration did not cause cytotoxicity in MCF10A cell lines. At higher concentrations (250 μg mL ), the nanoparticles showed the lowest cytotoxicity (17%). These findings suggest that C. aromaticus-mediated TiO NPs have significant biomedical applications. However, in-vivo studies are needed to learn more about their (C. aromaticus-mediated TiO NPs) potential biomedical applications.