A comprehensive review and comparison of L-tryptophan biosynthesis in Saccharomyces cerevisia e and Escherichia coli

L-tryptophan and its derivatives are widely used in the chemical, pharmaceutical, food, and feed industries. Microbial fermentation is the most commonly used method to produce L-tryptophan, which calls for an effective cell factory. The mechanism of L-tryptophan biosynthesis in , the widely used producer of L-tryptophan, is well understood. also plays a significant role in the industrial production of biochemicals. Because of its robustness and safety, is favored for producing pharmaceuticals and food-grade biochemicals. However, the biosynthesis of L-tryptophan in has been rarely summarized. The synthetic pathways and engineering strategies of L-tryptophan in and have been reviewed and compared in this review. Furthermore, the information presented in this review pertains to the existing understanding of how L-tryptophan affects stress fitness, which could aid in developing a novel plan to produce more resilient industrial yeast and cell factories.