lncNALT knockdown ameliorates hypertensive retinopathy via PTEN/PI3K/AKT pathway
This study aimed to explore the role of the long non-coding RNA NOTCH1-associated lncRNA in T cell acute lymphoblastic leukemia (lncNALT) in the pathogenesis of hypertensive retinopathy (HR). LncNALT expression levels were determined using reverse transcription-quantitative polymerase chain reaction...
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Veröffentlicht in: | Bioengineered 2022-12, Vol.13 (7-12), p.15003-15012 |
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Zusammenfassung: | This study aimed to explore the role of the long non-coding RNA NOTCH1-associated lncRNA in T cell acute lymphoblastic leukemia (lncNALT) in the pathogenesis of hypertensive retinopathy (HR). LncNALT expression levels were determined using reverse transcription-quantitative polymerase chain reaction. The effects of lncNALT knockdown on the viability, proliferation, migration, and invasion of human retinal microvascular endothelial cells (RMECs) were determined via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, 5-ethynyl-2'-deoxyuridine staining, and Transwell assays. Protein expression levels were determined using western blotting. We found that lncNALT expression levels were increased in RMECs treated with hydrogen peroxide (H
2
O
2
), while the knockdown of lncNALT rescued the viability, proliferation, migration, and invasion of RMECs treated with H
2
O
2
. Moreover, lncNALT interacted with ELAV like RNA binding protein 1 to affect the phosphatase and tensin homolog (PTEN) expression. Knockdown of lncNALT enhanced the viability, proliferation, migration, and invasion of RMECs via the PTEN/phosphoinositide 3-kinase (PI3K)/serine-threonine kinase (AKT) pathway. Taken together, knockdown of lncNALT enhanced the viability, proliferation, migration, and invasion of RMECs via the PTEN/PI3K/AKT pathway, suggesting that lncNALT could be a potential therapeutic target for patients with HR.
lncNALT interacts with HuR to increase the stability and expression levels of the PTEN
lncNALT regulates HR via the PTEN/PI3K/AKT pathway
lncNALT may be a potential diagnostic biomarker for HR |
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ISSN: | 2165-5979 2165-5987 |
DOI: | 10.1080/21655979.2023.2180591 |