Mentha rotundifolia (L.) Huds. aqueous extract attenuates H 2 O 2 induced oxidative stress and neurotoxicity

Oxidative stress plays a causal role in neurodegenerative diseases. The aim of this study is to evaluate the antioxidant and neuroprotective effects of (L.) Huds ( ), a widely used Moroccan plant in traditional medicine. The chemical composition of aqueous extract was analyzed by liquid chromatography coupled to mass spectrometry (LC-MS). 2,2-diphenyl 1-picrylhydrazyl (DPPH) and 2,2'-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS ) assays were used to assess its antioxidant activity. H O was utilized to induce oxidative stress and neurotoxicity . Behavioral changes were evaluated using Open Field, Y-maze and Rotarod tests. Hyperalgesia was assessed using the tail immersion test. The LC-MS/MS analysis revealed high content of kaempferol glucuronide (85%) at the extract. IC values of the DPPH and ABTS were 26.47 and 41.21 μg/mL, respectively. Pre-treatments with extract attenuated the behavioral changes induced by H O . In addition, the latency of tail withdrawal increased significantly in the treated groups suggesting central analgesic effect of extract. Moreover, the extract attenuated the deleterious effects of H O and improved all liver biomarkers. The obtained results suggested that had remarkable antioxidant and neuroprotective effects and may prevent oxidative stress related disorders.