Antiproliferative and apoptotic potential of methotrexate lipid nanoparticle in murine breast cancer model

To evaluate the efficacy of novel methotrexate-loaded nanoparticles (MTX-NPs) and in the treatment of breast cancer. MTX-NPs were tested for cellular uptake, cell viability, cell cycle, cellular wound migration and changes in tumor volume using characterized NPs. The solid lipid NPs (SLNPs) showed strong cellular uptake, increased apoptosis, controlled cytotoxicity at lower IC of methotrexate and a sizable reduction in tumor burden. MTX-NP oral formulation can be a promising candidate in breast cancer treatment with improved cellular uptake and efficacy.