Multiple-turnover single nucleotide primer extension reactions to detect 8-oxo-2′-deoxyguanosine in DNA

The identification of the position of 8-oxo-2′-deoxyguanosine (8-oxo-dG) in DNA is important to clarify the pathogenesis of many diseases. We herein developed a purine-1,3-diazaphenoxazine triphosphate (dPdapTP) and described the first example of detecting the presence of 8-oxo-dG by amplifying it several hundred times after the multiple-turnover single nucleotide primer extension reactions. An artificial nucleic acid triphosphate was successfully developed that was possible to amplify the positional information of the damaged nucleic acid of 8-oxo-dG in DNA several hundred times.