A simple and rapid method to assay SARS-CoV-2 RNA based on a primer exchange reaction

A simple and rapid method to assay SARS-CoV-2 RNA based on a primer exchange reaction

A simple method is proposed in this work for the detection of SARS-CoV-2 RNA based on a primer exchange reaction (PER). By ingeniously integrating the PER cascade and CRISPR/cas12a system, this method can achieve convenient detection of the target RNA in 40 min and distinguish a single-base mutation...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2022-04, Vol.58 (28), p.4484-4487
Hauptverfasser: Li, Dayong, Duan, Chengjie, Cheng, Wenting, gong, Youjing, Yao, Yanheng, Wang, Xiaoping, Wang, Zhongyun, Xiang, Yang
Format: Artikel
Sprache:eng
Schlagworte:
Mutation
Severe acute respiratory syndrome coronavirus 2
Target detection
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Zusammenfassung:A simple method is proposed in this work for the detection of SARS-CoV-2 RNA based on a primer exchange reaction (PER). By ingeniously integrating the PER cascade and CRISPR/cas12a system, this method can achieve convenient detection of the target RNA in 40 min and distinguish a single-base mutation from the target sequence, demonstrating its superior analytical performance. Integrating the PER cascade and CRISPR/cas12 system for the rapid detection of SARS-CoV-2 RNA.
ISSN:1359-7345
1364-548X
DOI:10.1039/d2cc00488g