Response of Saponaria officinalis L. hairy roots to the application of TiO 2 nanoparticles in terms of production of valuable polyphenolic compounds and SO6 protein

Saponaria officinalis L. is a perennial plant from the Caryophyllaceae family whose various parts are used in traditional medicine as the treatment agent of skin diseases, blood purifier, diuretic, sudorific, and bile purifier. The cultivation system of hairy roots is a proper alternative for improving the valuable pharmaceutical compounds production compared to other in-vitro methods. The extensive nanotechnology applications in hairy roots cultivation is a sustainable production foundation to produce such active elements. In this study, the effect of various concentrations of titanium dioxide nanoparticles (TiO NPs) (0, 10, 20, 30, 50 mg L ) with two treatments (24 and 48 h) was examined on the growth level, antioxidant capacity, total phenol and flavonoid contents, antioxidant enzyme activities, certain polyphenol compounds and SO6 protein in hairy roots of S. officinalis. According to the results, the maximum (3.09 g) and minimum (0.96 g) fresh weight (FW) of hairy roots were observed in treated culture media with 10 and 20 mg L of TiO NPs after 24 and 48 h of exposure times, respectively. The highest rate of total phenol (9.79 mg GLA g FW) and total flavonoid contents (1.06 mg QE g FW) were obtained in the treated hairy roots with 50 and 30 mg L of nano elicitor in 24 and 48 h of treatments, respectively. The maximum level of most polyphenols, such as rosmarinic acid, cinnamic acid, and rutin, was produced in 24 h of treatment. The use of TiO NP for 48 h with 50 mg L concentration showed the highest production level of SO6 protein.