3D print Platelet-rich plasma loaded scaffold with sustained cytokine release for bone defect repair

The combination of three-dimensionally (3D) printed scaffold materials and various cytokines can achieve the purpose of tissue reconstruction more efficiently. In the current study, we prepared Platelet-rich plasma (PRP)/gelatin microspheres combined with 3D printed polycaprolactone (PCL)/β-tricalcium phosphate (β-TCP) scaffolds to solve the key problem that PRP cannot be released under control and the release time is too short, and thus better promote bone repair. Consequently, the composite scaffold displayed a good mechanical property and sustained cytokine release for approximately 3 weeks. Additionally, increased survival, proliferation, migration and osteogenic and angiogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) were observed compared with the control groups. The in vivo study demonstrated that the composite scaffold with PRP/gelatin microspheres led to greater positive effects in promoting large bone defect repair. In conclusion, in the present study a new type of PRP long-term sustained-release composite scaffold material was constructed that effectively improved the survival, proliferation, and differentiation of cells in the transplanted area, thereby better promoting the repair of large bone defects.