Interaction between Chitosan and Chloroquine against Plasmodium berghei and P. falciparum Using In-Vivo and In-Vitro Tests

The use of antimalarial drugs with number of compounds in combination form may potentiate each other's activity. This study was conducted in the School of Public Health, Tehran University of Medical Sciences, Tehran, Iran in 2018. It was based on two methods including in vivo and in vitro tests with aim of considering interaction between chitosan and chloroquine against and parasites using different ratios of the agents with ED50 and IC50 baselines. Administrating 10 and 20 mg/kg (mouse body weight) of chitosan alone to the . -infected mice up to 4 successive days resulted in 37% and 45% inhibition of respectively, while employing the compound with chloroquine in combination form with ratios of 90/10 and 70/30 (chloroquine/chitosan) had a considerable potentiation including 71.58% and 83.85% inhibition effectiveness against Moreover, 20 mg/L (CCM) concentration of chitosan alone could eliminate 69.55% of in culture medium while in combination with chloroquine in ratios of 90/10 (chloroquine/chitosan) had considerable potentiation including 79.14% inhibition effectiveness. Mean survival time of those mice received combination therapy in ratios of 90/10 and 70/30 (chloroquine/chitosan) was longer than those took up mono therapy of either chloroquine or chitosan based on their ED50 doses. Interaction between chloroquine and chitosan showed considerable potentiation in combination form against either or using in vivo and in vitro tests respectively. Meanwhile, interaction between the above mentioned agents resulted in a notable survival time for those -infected mice treated with the combination.