Generation of Reactive Oxygen Species by Human Whole Blood Cells Exposed to Iron Oxide Magnetic Nanoparticles Coated with Different Shells

We compared dose-dependent influence of magnetic nanoparticles (MNP) coated with different shells on ROS generation during incubation with whole human blood. ROS generation was evaluated using luminol dependent chemiluminescence. MNP with polylactide shell in concentrations of 0.2-2.0 mg/ml had no effect on spontaneous chemiluminescence, while MNP with polysaccharide shell induced a dose dependent enhanced spontaneous chemiluminescence that increased during incubation (1-3 h). MNP with albumin shell in concentrations of 0.2 mg/ml gradually enhanced spontaneous chemiluminescence during incubation, but increasing the concentration neutralized this effect.