Continuous, On-Line DNA Sequencing Using Oligodeoxynucleotide Primers with Multiple Fluorophores

A method for sequencing DNA by using a difluoresceinated primer and laser excitation is described. Dideoxy protocols have been determined that provide sequences for 600 bases starting with base 1 with

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Bibliographische Detailangaben
Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1988-08, Vol.85 (15), p.5610-5614
Hauptverfasser:	Brumbaugh, John A., Middendorf, Lyle R., Grone, Daniel L., Ruth, J. L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Autoradiography Base Sequence Chemical Phenomena Chemistry Deoxyuridine - analogs & derivatives DNA DNA - genetics Electrophoresis Electrophoresis, Polyacrylamide Gel Fluorescence Fluorescent Dyes fluorescent probes Gels Humans Image Processing, Computer-Assisted Imaging Lasers Microscopes Oligonucleotides Optical filters Predetermined motion time systems Sequencing Software
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Beschreibung
Zusammenfassung:	A method for sequencing DNA by using a difluoresceinated primer and laser excitation is described. Dideoxy protocols have been determined that provide sequences for 600 bases starting with base 1 with
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.85.15.5610