A Novel Fat Making Strategy for Adipose-Derived Progenitor Cells Enrichment Fat Improves Fat Graft Survival

A low survival rate is one of the main challenges in fat grafting. This study aimed to evaluate whether the microfat obtained using a novel strategy promoted the survival and retention of fat grafts. A 5 mm diameter blunt tip cannula with larger side holes (~30 mm 2 per hole) was used to obtain macrofat. Subsequently, a novel strategy based on a newly invented extracorporeal cutting device was used to cut the macrofat into microfat, which was named adipose-derived progenitor cells enrichment fat (AER fat); Coleman fat was used as the control. Aliquots (0.5 mL) of both groups of fat were transplanted into 10 nude mice and analyzed 10 weeks later. Western blotting, flow cytometry, and immunofluorescence were performed to assess the AER characteristics and underlying mechanisms. The retention rate of fat grafts in AER fat-treated animals was significantly higher than that in the Coleman group (54.6±13% vs. 34.8±9%, P