Chemo-enzymatic synthesis of Lacto-N-biose I catalyzed by β-1,3-galactosidase from Bacillus circulans using 4,6-dimethoxy-1,3,5-triazin-2-yl β-galactopyranoside as a glycosyl donor

ABSTRACT Chemo-enzymatic synthesis of lacto-N-biose I (LNB) catalyzed by β-1,3-galactosidase from Bacillus circulans (BgaC) has been developed using 4,6-dimethoxy-1,3,5-triazin-2-yl β-galactopyranoside (DMT-β-Gal) and GlcNAc as the donor and acceptor substrates, respectively. BgaC transferred the Ga...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2021-06, Vol.85 (7), p.1716-1719
Hauptverfasser:	Ohnuma, Takayuki, Taku, Tomoki, Nagatani, Takeshi, Horii, Atsushi, Imaoka, Shun, Tanaka, Tomonari
Format:	Artikel
Sprache:	eng
Schlagworte:	Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Chemistry Chemistry, Applied Food Science & Technology Life Sciences & Biomedicine Physical Sciences Science & Technology
Online-Zugang:	Volltext
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Zusammenfassung:	ABSTRACT Chemo-enzymatic synthesis of lacto-N-biose I (LNB) catalyzed by β-1,3-galactosidase from Bacillus circulans (BgaC) has been developed using 4,6-dimethoxy-1,3,5-triazin-2-yl β-galactopyranoside (DMT-β-Gal) and GlcNAc as the donor and acceptor substrates, respectively. BgaC transferred the Gal moiety to the acceptor, giving rise to LNB. The maximum yield of LNB was obtained at the acceptor : donor substrate ratio of 1:30.
ISSN:	0916-8451 1347-6947 1347-6947
DOI:	10.1093/bbb/zbab071