Transient Depletion of CD4(+) Cells Induces Remodeling of the TCR Repertoire in Gastrointestinal Cancer

Antibody-mediated transient depletion of CD4(+) cells enhances the expansion of tumor-reactive CD8(+) T cells and exhibits robust antitumor effects in preclinical and clinical studies. To investigate the clonal T-cell responses following transient CD4(+) cell depletion in patients with cancer, we co...

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Veröffentlicht in:Cancer immunology research 2021-06, Vol.9 (6), p.624-636
Hauptverfasser: Aoki, Hiroyasu, Ueha, Satoshi, Shichino, Shigeyuki, Ogiwara, Haru, Shitara, Kohei, Shimomura, Manami, Suzuki, Toshihiro, Nakatsura, Tetsuya, Yamashita, Makiko, Kitano, Shigehisa, Kuroda, Sakiko, Wakabayashi, Masashi, Kurachi, Makoto, Ito, Satoru, Doi, Toshihiko, Matsushima, Kouji
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Sprache:eng
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Zusammenfassung:Antibody-mediated transient depletion of CD4(+) cells enhances the expansion of tumor-reactive CD8(+) T cells and exhibits robust antitumor effects in preclinical and clinical studies. To investigate the clonal T-cell responses following transient CD4(+) cell depletion in patients with cancer, we conducted a temporal analysis of the T-cell receptor (TCR) repertoire in the first-in-human clinical trial of IT1208, a defucosylated humanized monoclonal anti-CD4. Transient depletion of CD4(+) cells promoted replacement of T-cell clones among CD4(+) and CD8(+) T cells in the blood. This replacement of the TCR repertoire was associated with the extent of CD4(+) T-cell depletion and an increase in CD8(+) T-cell count in the blood. Next, we focused on T-cell clones overlapping between the blood and tumor in order to track tumor-associated T-cell clones in the blood. The total frequency of blood-tumor overlapping clones tended to increase in patients receiving a depleting dose of anti-CD4, which was accompanied by the replacement of overlapping clones. The greater expansion of CD8(+) overlapping clones was commonly observed in the patients who achieved tumor shrinkage. These results suggested that the clonal replacement of the TCR repertoire induced by transient CD4(+) cell depletion was accompanied by the expansion of tumor-reactive T-cell clones that mediated antitumor responses. Our findings propose beneficial remodeling of the TCR repertoire following transient CD4(+) cell depletion and provide novel insight into the antitumor effects of monoclonal anti-CD4 treatment in patients with cancer.
ISSN:2326-6066
2326-6074
DOI:10.1158/2326-6066.CIR-20-0989