Schlegelella koreensis sp. nov., isolated from evaporator core of automobile air conditioning system

A white-coloured, aerobic, and rod-shaped bacterium, designated strain ID0723 T was isolated from evaporator core of automobile air conditioning system. The strain was Gram-stain-negative, catalase positive, oxidase negative, and grew at pH 5.5–9.5, at temperature 18–37 °C, and at 0–2.0% (w/v) NaCl concentration. The phylogenetic analysis and 16S rRNA gene sequence data revealed that the strain ID0723 T was affiliated to the genus Schlegelella , with the closest phylogenetic member being Schlegelella brevitalea DSM 7029 T (98.1% sequence similarity). The chemotaxonomic features of strain ID0723 T were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine as the main polar lipids; Q-8 as an only ubiquinone; and summed feature 3 (C 16:1 ω 7 c and/or C 16: 1 ω 6 c ), C 16:0 , and summed feature 8 (C 18:1 ω 7c/or C 18:1 ω6 c) as the major fatty acids. The average nucleotide identity (ANI) and in silico DNA–DNA hybridization values between strain ID0723 T and S. brevitalea DSM 7029 T were 74.8% and 20.0%, respectively, which were below the cut-off values of 95% and 70%, respectively. The DNA G + C content was 69.9 mol%. The polyphasic taxonomic data clearly indicated that strain ID0723 T represents a novel species in the genus Schlegelella for which the name Schlegelella koreensis sp. nov. is proposed, with the type strain ID0723 T (= KCTC 72731 T = NBRC 114611 T ).