Polyphenol-rich vinegar extract regulates intestinal microbiota and immunity and prevents alcohol-induced inflammation in mice

[Display omitted] •Vinegar extract was rich in polyphenol.•Vinegar extract modulate gut microbiota and immunity in ethanol-treated mice.•Vinegar extract attenuated LPS-mediated inflammation in ethanol-treated mice.•Vinegar extract prevented ethanol-induced inflammation via gut-liver axis. Zhenjiang aromatic vinegar (ZAV), a traditional fermented food in China, is rich in polyphenols with health-beneficial effects. In this study, vinegar extract ameliorated ethanol-induced liver injury by reducing the levels of oxidative stress biomarkers. In addition, vinegar extract regulated gut microbiota composition and immune factors, and improved antimicrobial peptides (Reg3b and Reg3g) and intestinal homeostasis in ethanol-treated mice. Vinegar extract suppressed lipopolysaccharide (LPS)-mediated inflammatory response in the liver and gut of ethanol-treated mice. Moreover, Akkermansia, Lachnospiraceae_NK4A136_group and Bacteroidetes showed a positive correlation with intestinal immune factors and antimicrobial peptides, and a negative correlation with parameters of oxidative stress and inflammation. In contrast, Firmicutes, Proteobacteria, Bilophila and Butyricimonas showed the opposite correlation with these parameters. Our study provides a new sight into vinegar extract for the prevention of ethanol-induced liver damage via modulation of gut-liver axis.