Integrating quantitative proteomics with accurate genome profiling of transcription factors by greenCUT&RUN

Abstract Genome-wide localization of chromatin and transcription regulators can be detected by a variety of techniques. Here, we describe a novel method ‘greenCUT&RUN’ for genome-wide profiling of transcription regulators, which has a very high sensitivity, resolution, accuracy and reproducibili...

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Veröffentlicht in:	Nucleic acids research 2021-05, Vol.49 (9), p.e49-e49
Hauptverfasser:	Nizamuddin, Sheikh, Koidl, Stefanie, Bhuiyan, Tanja, Werner, Tamara V, Biniossek, Martin L, Bonvin, Alexandre M J J, Lassmann, Silke, Timmers, HThMarc
Format:	Artikel
Sprache:	eng
Schlagworte:	Binding Sites CCAAT-Binding Factor - genetics CCAAT-Binding Factor - metabolism Genomics - methods Green Fluorescent Proteins - genetics Green Fluorescent Proteins - immunology HeLa Cells Humans Mass Spectrometry Methods Online Proteomics - methods Proto-Oncogene Proteins c-fos - genetics Proto-Oncogene Proteins c-fos - metabolism Recombinant Fusion Proteins - analysis Single-Domain Antibodies TATA-Box Binding Protein - genetics TATA-Box Binding Protein - metabolism Transcription Factors - metabolism
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