Arabidopsis N 6 -methyladenosine reader CPSF30-L recognizes FUE signals to control polyadenylation site choice in liquid-like nuclear bodies

The biological functions of the epitranscriptomic modification N -methyladenosine (m A) in plants are not fully understood. CPSF30-L is a predominant isoform of the polyadenylation factor CPSF30 and consists of CPSF30-S and an m A-binding YTH domain. Little is known about the biological roles of CPS...

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Veröffentlicht in:Molecular plant 2021-04, Vol.14 (4), p.571
Hauptverfasser: Song, Peizhe, Yang, Junbo, Wang, Chunling, Lu, Qiang, Shi, Linqing, Tayier, Subiding, Jia, Guifang
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Sprache:eng
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Zusammenfassung:The biological functions of the epitranscriptomic modification N -methyladenosine (m A) in plants are not fully understood. CPSF30-L is a predominant isoform of the polyadenylation factor CPSF30 and consists of CPSF30-S and an m A-binding YTH domain. Little is known about the biological roles of CPSF30-L and the molecular mechanism underlying its m A-binding function in alternative polyadenylation. Here, we characterized CPSF30-L as an Arabidopsis m A reader whose m A-binding function is required for the floral transition and abscisic acid (ABA) response. We found that the m A-binding activity of CPSF30-L enhances the formation of liquid-like nuclear bodies, where CPSF30-L mainly recognizes m A-modified far-upstream elements to control polyadenylation site choice. Deficiency of CPSF30-L lengthens the 3' untranslated region of three phenotypes-related transcripts, thereby accelerating their mRNA degradation and leading to late flowering and ABA hypersensitivity. Collectively, this study uncovers a new molecular mechanism for m A-driven phase separation and polyadenylation in plants.
ISSN:1752-9867
DOI:10.1016/j.molp.2021.01.014