Promoter P PSP 1-5 - BnPSP-1 From Ramie ( Boehmeria nivea L. Gaud.) Can Drive Phloem-Specific GUS Expression in Arabidopsis thaliana

Isolation of phloem-specific promoters is one of the basic conditions for improving the fiber development and resistance of ramie phloem using genetic engineering. In this study, we isolated a ramie endogenous promoter (named ) and analyzed the function of its truncated fragments in Arabidopsis. The results show that can drive the GUS reporter gene to be specifically expressed in the veins of Arabidopsis. After hormone and simulated drought treatment of the independent Arabidopsis lines carrying and its truncated fragments, only (-600 to -1 bp region of ) is stably expressed and exhibits phloem specificity. Our findings suggest that can be used as a phloem specific promoter for further research.