Toxicological effects of Ag 2 O and Ag 2 CO 3 doped TiO 2 nanoparticles and pure TiO 2 particles on zebrafish (Danio rerio)

In this study, the toxic effects of silver oxide and silver carbonate doped TiO nanoparticles (Ag O-TiO NPs and Ag CO -TiO NPs), TiO nanoparticles (TiO NPs), and bulk TiO on gene expression, lipid peroxidation, genotoxicity, and histological alterations in zebrafish (Danio rerio) was assessed. The physicochemical properties of the synthesized nanoparticles were evaluated by X-ray diffraction (XRD), Scanning Electron Microscope (SEM), diffuse reflectance spectroscopy (DRS), dynamic light scattering (DLS), and Zeta potential analyses. TiO NPs after doping with Ag showed shift to higher wavelengths and decrease of band gap energy. Also, remarkable reduction in the size of Ag-doped TiO NPs in comparison with the TiO NPs was observed. According to our results, acute toxicity increased in the order of bulk TiO