Agriterribacter humi gen. nov., sp. nov., a novel bacterium of the family Chitinophagaceae isolated from soil of a farming field

A Gram-stain-negative bacterium, designated strain YJ03(T). was isolated from a spinach farming field soil at Shinan in Korea. Strain YJ03(T) was found to be an aerobic, non-motile and non-spore-forming bacterium which can grow at 10-33 degrees C (optimum. 25-28 degrees C), at pH 6.6-9.5 (optimum, pH 7.0-7.5) and at salinities of 0-1.0% (w/v) NaCl (optimum, 0% NaCl). Sequence similarities of the 16S rRNA gene of strain YJ03(T) with the closely related relatives were in the range 93.9-92.2%, and the results of phylogenomic analysis indicated that strain YJ03(T) was clearly separated from species of the genera in the family Chitinophagaceae. showing average nucleotide identity values of 68.8-64.3%. The predominant isoprenoid quinone was identified as MK-7 and the major fatty acids were iso-C-15:0, iso-C-17:0 3-OH and an unidentified fatty acid with an equivalent chain-length of 13.565. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, four unidentified aminolipids and six unidentified lipids. The G+C content of the genome was determined to be 41.8 mol%. On the basis of phenotypic and chemotaxonomic properties and phylogenetic and phylogenomic analyses using 16S rRNA gene sequences and whole-genome sequences in this study, strain YJ03(T) is considered to represent a novel species of a new genus in the family Chitinophagaceae, for which the name Agriterribacter humi gen. nov., sp. nov., is proposed. The type strain of Agriterribacter humi is YJ03(T) (=KACC 19548(T)=NBRC 113195(T)).