The activation of D 2 and D 3 receptor subtypes inhibits pathways mediating primary afferent depolarization (PAD) in the mouse spinal cord

Somatosensory information can be modulated at the spinal cord level by primary afferent depolarization (PAD), known to produce presynaptic inhibition (PSI) by decreasing neurotransmitter release through the activation of presynaptic ionotropic receptors. Descending monoaminergic systems also modulat...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Neuroscience letters 2020-09, Vol.736, p.135257
Hauptverfasser: Milla-Cruz, Jonathan J, Mena-Avila, Elvia, Calvo, Jorge R, Hochman, Shawn, Villalón, Carlos M, Quevedo, Jorge N
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Somatosensory information can be modulated at the spinal cord level by primary afferent depolarization (PAD), known to produce presynaptic inhibition (PSI) by decreasing neurotransmitter release through the activation of presynaptic ionotropic receptors. Descending monoaminergic systems also modulate somatosensory processing. We investigated the role of D -like and D -like receptors on pathways mediating PAD in the hemisected spinal cord of neonatal mice. We recorded low-threshold evoked dorsal root potentials (DRPs) and population monosynaptic responses as extracellular field potentials (EFPs). We used a paired-pulse conditioning-test protocol to assess homosynaptic and heterosynaptic depression of evoked EFPs to discriminate between dopaminergic effects on afferent synaptic efficacy and/or on pathways mediating PAD, respectively. DA (10 μM) depressed low-threshold evoked DRPs by 43 %, with no effect on EFPs. These depressant effects on DRPs were mimicked by the D -like receptor agonist quinpirole (35 %). Moreover, by using selective antagonists at D -like receptors (encompassing the D , D , and D subtypes), we found that the D and D receptor subtypes participate in the quinpirole depressant inhibitory effects of pathways mediating PAD.
ISSN:1872-7972
DOI:10.1016/j.neulet.2020.135257