Rabbit antisera to enterobacteriaceae isolated from HLA-B27 positive patients with ankylosing spondylitis (AS) are lytic for the mononuclear cells of AS patients

Rabbit antisera to enterobacteriaceae isolated from HLA-B27 positive patients with ankylosing spondylitis (AS) are lytic for the mononuclear cells of AS patients

Vaccines prepared from Gram-negative bacteria isolated from the stools of HLA-B27 positive AS patients were used to immunize rabbits. Three of the sera obtained were lytic in vitro for the mononuclear cells of HLA-B27 positive AS patients. One of these sera discriminated between AS patients and heal...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:British journal of rheumatology 1988, Vol.27 Suppl 2, p.58
Hauptverfasser: Beukelman, C J, van Leeuwen, A, Aerts, P C, van Kregten, E, van Bree, S, van Rood, J J, Willers, J M, van Dijk, H
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cytotoxicity Tests, Immunologic
Enterobacteriaceae - immunology
HLA Antigens - immunology
HLA-B27 Antigen
Humans
Immune Sera - toxicity
Leukocytes, Mononuclear - drug effects
Rabbits - immunology
Spondylitis, Ankylosing - blood
Spondylitis, Ankylosing - immunology
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Vaccines prepared from Gram-negative bacteria isolated from the stools of HLA-B27 positive AS patients were used to immunize rabbits. Three of the sera obtained were lytic in vitro for the mononuclear cells of HLA-B27 positive AS patients. One of these sera discriminated between AS patients and healthy HLA-B27 positive individuals. Cytolysis was determined in an automated, non-radioactive assay based on the release of carboxyfluorescein diacetate and the incorporation of propidium iodide.
ISSN:0263-7103