Molecular characterization, expression analysis of 14-3-3 beta/alpha and the effect of RNA interference on ion transporter protein Na+-K+-ATPase, Na+–H+-exchanger and CFTR in turbot (Scophthalmus maximus)

To understand the role of 14–3-3 beta/alpha in hypoosmotic regulation of turbot (Scophthalmus maximus), we characterized the 14–3-3 beta/alpha gene and analyzed the tissue distribution and its gene transcriptional patterns in the main expressed tissues under low salt stress. The 14–3-3 beta/alpha cDNA is 892 bp in length, incorporating an ORF of 774 bp with a putative primary structure of 257 residues. The deduced amino acid sequences shared highly conserved structures with other eukaryotes. Quantitative real-time PCR results showed that the 14–3-3 beta/alpha transcripts were widely expressed in various tissues of turbot, with most abundant in the gill (P