Improvement of a miRNA inhibitor by intracellular selection

Sequences surrounding the miRNA binding domain of the miRNA inhibitor LidNA were selected intracellularly. The library was transfected into cells, and then, inhibitors that were associated with argonaute 2 were selected. The potent inhibitors were slowly degraded intracellularly, while the lower-act...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2020-07, Vol.84 (7), p.1451-1454
Hauptverfasser:	Tachibana, Akira, Yamamoto, Aiko
Format:	Artikel
Sprache:	eng
Schlagworte:	Ago2 Argonaute Proteins - metabolism Base Sequence DNA Probes - chemistry DNA Probes - genetics DNA Probes - pharmacology DNA, Single-Stranded - chemistry DNA, Single-Stranded - metabolism HEK293 Cells Humans LidNA MicroRNAs - antagonists & inhibitors MicroRNAs - genetics MicroRNAs - metabolism miRNA miRNA inhibitor Oligonucleotides - pharmacology RNA-Binding Motifs Transfection
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Beschreibung
Zusammenfassung:	Sequences surrounding the miRNA binding domain of the miRNA inhibitor LidNA were selected intracellularly. The library was transfected into cells, and then, inhibitors that were associated with argonaute 2 were selected. The potent inhibitors were slowly degraded intracellularly, while the lower-activity inhibitors were rapidly degraded. A combination of the selected sequences surrounding the miRNA binding domain enhanced miRNA inhibitory activity. LidNA: DNA that puts a lid on miRNA function; LNA: locked nucleic acid; Ago2: argonaute 2; LNA: locked nucleic acid
ISSN:	0916-8451 1347-6947
DOI:	10.1080/09168451.2020.1743167