Diversity of Mycoplasma hyopneumoniae strains from pigs across Ontario, Canada

Mycoplasma hyopneumoniae causes highly contagious swine enzootic pneumonia worldwide. It has been reported that highly diversified M. hyopneumoniae strains exist in different parts of the world. We found p146 gene sequencing analysis, an affordable and simple-to-perform typing method, to be specific and highly sensitive when applied to the molecular typing of 113 M. hyopneumoniae–positive clinical samples directly without culture. The samples were submitted to the Animal Health Laboratory at the University of Guelph (Ontario, Canada) during 2009–2017 from 40 different geographic areas in Ontario. Using a previously described criterion of grouping strains with