A sensitive, wide-ranging comprehensive toxicity indicator based on microbial fuel cell

[Display omitted] •The average current inhibition rate, a toxicity indicator was proposed.•Good linear relationships were found between ACIR and toxic compounds.•PCoA was performed on microbial community in anode chamber.•A better consistency was got between ACIR and electrogenic bacteria. An innovative indicator for toxicity detection based on microbial fuel cells, average current inhibition rate (ACIR) was proposed. It was applied to the toxicity evaluation of three typical specific pollutants in petrochemical wastewater including copper(II), 2,4-dichlorophenol (2,4-DCP) and pyridine. ACIR which considered the entire process of toxic effects was proved to be more sensitive and wide-ranging than the conventional indicators. The linear detection ranges were 0.3–100 mg/L of copper(II), 0.4–1000 mg/L of 2,4-DCP, and 0.1–1000 mg/L of pyridine. The median effective concentrations of the three toxicants were 34.32, 36.18 and above 1000 mg/L, respectively. By contrast, using a conventional indicator such as the voltage inhibition rate, the calculation results consistently change with the exposure time. Based on the response time, the toxicity will be difficult to distinguish under high concentrations. An analysis of the microbial community in anode chamber showed that electrogenic bacteria such as Geobacter and Arcobacter significantly decreased with 2,4-DCP and pyridine under all tested concentrations. A principal component analysis was conducted, the results of which showed that the microbial community shifted from left to right with the increase concentration of copper(II) and 2,4-DCP. An increase of ACIR was noticed to be in accordance with the reduction of electrogenic bacteria.