IFNγ induces epigenetic programming of human T-bet hi B cells and promotes TLR7/8 and IL-21 induced differentiation
Although B cells expressing the IFNγR or the IFNγ-inducible transcription factor T-bet promote autoimmunity in Systemic Lupus Erythematosus (SLE)-prone mouse models, the role for IFNγ signaling in human antibody responses is unknown. We show that elevated levels of IFNγ in SLE patients correlate wit...
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Veröffentlicht in: | eLife 2019-05, Vol.8 |
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Hauptverfasser: | , , , , , , , , , , , , , , , , , , , , |
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Sprache: | eng |
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Zusammenfassung: | Although B cells expressing the IFNγR or the IFNγ-inducible transcription factor T-bet promote autoimmunity in Systemic Lupus Erythematosus (SLE)-prone mouse models, the role for IFNγ signaling in human antibody responses is unknown. We show that elevated levels of IFNγ in SLE patients correlate with expansion of the T-bet expressing IgD
CD27
CD11c
CXCR5
(DN2) pre-antibody secreting cell (pre-ASC) subset. We demonstrate that naïve B cells form T-bet
pre-ASCs following stimulation with either Th1 cells or with IFNγ, IL-2, anti-Ig and TLR7/8 ligand and that IL-21 dependent ASC formation is significantly enhanced by IFNγ or IFNγ-producing T cells. IFNγ promotes ASC development by synergizing with IL-2 and TLR7/8 ligands to induce genome-wide epigenetic reprogramming of B cells, which results in increased chromatin accessibility surrounding IRF4 and BLIMP1 binding motifs and epigenetic remodeling of
and
loci. Finally, we show that IFNγ signals poise B cells to differentiate by increasing their responsiveness to IL-21. |
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ISSN: | 2050-084X |