P435 HER2/neu-derived peptide conjugated to liposomes containing DOPE as an effective prophylactic vaccine formulation for breast cancer

The present study was aimed to develop an effective nanoliposomal vaccine delivery system with P435 HER2/neu-derived peptide conjugated to Maleimide-PEG2000-DSPE. The nanoliposome formulation composed of DSPC/DSPG/Chol/DOPE and monophosphoryl lipid A was used as an adjuvant. Liposomal formulations w...

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Veröffentlicht in:	Artificial cells, nanomedicine, and biotechnology nanomedicine, and biotechnology, 2019-12, Vol.47 (1), p.664-672
Hauptverfasser:	Farzad, Niloofar, Barati, Nastaran, Momtazi-Borojeni, Amir Abbas, Yazdani, Mona, Arab, Atefeh, Razazan, Atefeh, Shariat, Sheida, Mansourian, Mercedeh, Abbasi, Azam, Saberi, Zahra, Badiee, Ali, Jalali, Seyed Amir, Jaafari, Mahmoud Reza
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Anticancer properties Breast cancer Cancer vaccines Cancer Vaccines - chemistry Cancer Vaccines - immunology Cancer Vaccines - pharmacology Cell Line, Tumor cytotoxic T-cell response Drug delivery systems Enzyme-linked immunosorbent assay ErbB-2 protein Female Flow cytometry HER2/neu protein Immune response Immune system Immunization Lipid A Lipids Liposomes Mammary Neoplasms, Experimental - immunology Mammary Neoplasms, Experimental - prevention & control Mice, Inbred BALB C Monophosphoryl lipid A Nanoparticles - chemistry Nanoparticles - therapeutic use P435 peptide Peptide-conjugated nanoliposomes Peptides Peptides - chemistry Peptides - immunology Peptides - pharmacology Physical properties prophylactic vaccine Receptor, ErbB-2 - chemistry Receptor, ErbB-2 - immunology Receptor, ErbB-2 - therapeutic use Vaccines
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Zusammenfassung:	The present study was aimed to develop an effective nanoliposomal vaccine delivery system with P435 HER2/neu-derived peptide conjugated to Maleimide-PEG2000-DSPE. The nanoliposome formulation composed of DSPC/DSPG/Chol/DOPE and monophosphoryl lipid A was used as an adjuvant. Liposomal formulations were prepared and their physical properties were characterized. Anti-tumoral efficacy of formulations was evaluated by immunization of tumor-bearing BALB/c mice and the generated immune response was studied by using ELISpot and flow cytometry analysis. The results of the study demonstrated Lip + DOPE + P535 formulation caused the lowest tumor size and the longest survival time in TUBO mice model and could make it a promising candidate in developing effective vaccines against HER2-positive breast cancers.
ISSN:	2169-1401 2169-141X
DOI:	10.1080/21691401.2019.1576702