Two-dimensional SERS encoding method for on-bead peptide sequencing in high-throughput bioanalysis

We developed a ready-to-read on-bead peptide encoding method for high-throughput screening bioassays. With two-dimensional surface-enhanced Raman scattering nano-identifiers (2D-SERS IDs) which are concurrently labelled with two SERS codes (coupling steps and kinds of amino acid), we could possibly...

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Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2019-02, Vol.55 (18), p.27-273
Hauptverfasser:	Jeong, Sinyoung, Kang, Homan, Cha, Myeong Geun, Lee, Sung Gun, Kim, Jaehi, Chang, Hyejin, Lee, Yoon-Sik, Jeong, Dae Hong
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acids - chemistry Coding High-Throughput Screening Assays Nanoparticles - chemistry Peptides Peptides - analysis Peptides - chemistry Raman spectra Silicon Dioxide - chemistry Spectrum Analysis, Raman - methods
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	We developed a ready-to-read on-bead peptide encoding method for high-throughput screening bioassays. With two-dimensional surface-enhanced Raman scattering nano-identifiers (2D-SERS IDs) which are concurrently labelled with two SERS codes (coupling steps and kinds of amino acid), we could possibly generate more than 10 trillion codes with only 30 Raman label compounds. Sequence and kinds of amino acids of on-bead peptide can be ready-to-read by 2D-SERS identifiers for high-throughput screening.
ISSN:	1359-7345 1364-548X
DOI:	10.1039/c8cc10224d