Control of Steroid Synthesis in Adrenal Fasciculata Cells

The control of steroid synthesis in adrenal fasciculata cells is considered in terms of two types of control by ACTH: 1) control of cholesterol availability to inner mitochondrial cytochrome P-450scc. This process controls total steroid synthesis and is rapidly activated by ACTH. The several steps in cholesterol transfer are examined. 2) partitioning of metabolism by means of competition between enzymes for limiting amounts of steroid intermediates. Changes in such competition determine the ratio of steroid products from the adrenal cells. Such changes typically are a slower response to ACTH. A critical aspect of such competition is the modulation of multiple activity P-450 cytochromes: P-45017α (17α-hydroxylation and 17,20 lyase) and P-450211β (11α and 18-oxidases). Factors such as substrate binding, electron transfer steps and lipid environment are considered in addition to new enzyme synthesis. The ACTH stimulation of steroid synthesis in bovine adrenal cell primary cultures is examined as a model for both types of regulation.