Supramolecular protein cages constructed from a crystalline protein matrix

Protein crystals are formed via ordered arrangements of proteins, which assemble to form supramolecular structures. Here, we show a method for the assembly of supramolecular protein cages within a crystalline environment. The cages are stabilized by covalent cross-linking allowing their release via dissolution of the crystal. The high stability of the desiccated protein crystals allows cages to be constructed. Protein cages were constructed by cross-linking monomers in polyhedra crystals, followed by dissolution of the crystals.