Purification and Characterization of a Newly Identified Growth Factor Specific for Epithelial Cells

A growth factor specific for epithelial cells was identified in conditioned medium of a human embryonic lung fibroblast cell line, The factor, provisionally termed keratinocyte growth factor (KGF) because of its predominant activity on this cell type, was purified to homogeneity by a combination of...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1989-02, Vol.86 (3), p.802-806
Hauptverfasser: Rubin, Jeffrey S., Osada, Hiroyuki, Finch, Paul W., Taylor, William G., Rudikoff, Stuart, Aaronson, Stuart A.
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Sprache:eng
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Zusammenfassung:A growth factor specific for epithelial cells was identified in conditioned medium of a human embryonic lung fibroblast cell line, The factor, provisionally termed keratinocyte growth factor (KGF) because of its predominant activity on this cell type, was purified to homogeneity by a combination of ultrafiltration, heparin-Sepharose affinity chromatography, and hydrophobic chromatography on a C4 reversed-phase HPLC column. KGF was both acid and heat labile and consisted of a single polypeptide chain of ≈ 28 kDa. Purified KGF was a potent mitogen for epithelial cells, capable of stimulating DNA synthesis in quiescent BALB/MK epidermal keratinocytes by >500-fold with activity detectable at 0.1 nM and maximal at 1.0 nM. Lack of mitogenic activity on either fibroblasts or endothelial cells indicated that KGF possessed a target cell specificity distinct from any previously characterized growth factor. Microsequencing revealed an amino-terminal sequence containing no significant homology to any known protein. The release of this growth factor by human embryonic fibroblasts raises the possibility that KGF may play a role in mesenchymal simulation of normal epithelial cell proliferation.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.86.3.802