A water-soluble two-photon ratiometric triarylboron probe with nucleolar targeting by preferential RNA binding

A water-soluble two-photon ratiometric triarylboron probe with nucleolar targeting by preferential RNA binding

By functionalizing triarylboron with cyclen, we developed a two-photon fluorescence probe, TAB-2 , which can selectively bind RNA with a ratiometric readout. We tested TAB-2 in NIH/3T3 fibroblast cells, and demonstrated its capability in visualizing nucleoli and analyzing microenvironment polarity b...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2017-10, Vol.53 (83), p.11476-11479
Hauptverfasser: Liu, Jun, Zhang, Shilu, Zhang, Chenghua, Dong, Jun, Shen, Chengyi, Zhu, Jiang, Xu, Huajun, Fu, Mingkai, Yang, Guoqiang, Zhang, Xiaoming
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Sprache:eng
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Zusammenfassung:By functionalizing triarylboron with cyclen, we developed a two-photon fluorescence probe, TAB-2 , which can selectively bind RNA with a ratiometric readout. We tested TAB-2 in NIH/3T3 fibroblast cells, and demonstrated its capability in visualizing nucleoli and analyzing microenvironment polarity by two-photon and fluorescence-lifetime imaging microscopy. By functionalizing triarylboron with cyclen, we developed a water-soluble two-photon ratiometric triarylboron probe with nucleolar targeting by preferential RNA binding.
ISSN:1359-7345
1364-548X
DOI:10.1039/c7cc06045a