Genetic analysis of the gentamicin resistance region of pPH1JI and incorporation into a wide host range cloning vehicle

A region of the IncP plasmid pPH1JI encoding resistance to gentamicin, spectinomycin, and streptomycin was characterized by subcloning, deletion, and insertion mutagenesis. Approximate locations of these resistance determinants were established. A 1.6-kb HindIII-SphI segment of this region expresses...

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Veröffentlicht in:	Plasmid 1987-07, Vol.18 (1), p.84-88
1. Verfasser:	Rubin, R.A
Format:	Artikel
Sprache:	eng
Schlagworte:	ADN AGROBACTERIUM AGROBACTERIUM TUMEFACIENS ANTIBIOTICOS ANTIBIOTICS ANTIBIOTIQUE Biological and medical sciences Biotechnology CHEMICAL RESISTANCE Cloning, Molecular DNA DNA Restriction Enzymes Drug Resistance, Microbial DRUGS Escherichia coli - drug effects Escherichia coli - genetics Fundamental and applied biological sciences. Psychology GENE GENES Genetic engineering Genetic technics Gentamicins - pharmacology MEDICAMENT MEDICAMENTOS Methods. Procedures. Technologies PLASMIDE PLASMIDIOS PLASMIDS R Factors RESISTANCE AUX PRODUITS CHIMIQUES RESISTENCIA QUIMICA RHIZOBIUM RHIZOBIUM MELILOTI Vectors (cloning, transfer, expression). Insertion sequences and transposons
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Zusammenfassung:	A region of the IncP plasmid pPH1JI encoding resistance to gentamicin, spectinomycin, and streptomycin was characterized by subcloning, deletion, and insertion mutagenesis. Approximate locations of these resistance determinants were established. A 1.6-kb HindIII-SphI segment of this region expresses gentamicin resistance (Gmr) in Escherichia coli when inserted into various plasmid vectors; this DNA segment encodes a polypeptide of 17.5 kDa. Incorporation of this fragment into an IncP cloning vehicle produced a Gmr wide host range vector, pRAR209, which confers levels of Gmr comparable to those expressed by pPH1JI in E. coli, Agrobacterium tumefaciens, and Rhizobium meliloti.
ISSN:	0147-619X 1095-9890