Macrophage LTB 4 drives efficient phagocytosis of Borrelia burgdorferi via BLT1 or BLT2
Unresolved experimental Lyme arthritis in C3H 5-lipoxygenase (5-LOX) mice is associated with impaired macrophage phagocytosis of In the present study, we further investigated the effects of the 5-LOX metabolite, leukotriene (LT)B on phagocytosis of Bone marrow-derived macrophages (BMDMs) from 5-LOX...
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Veröffentlicht in: | Journal of lipid research 2017-03, Vol.58 (3), p.494 |
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Zusammenfassung: | Unresolved experimental Lyme arthritis in C3H 5-lipoxygenase (5-LOX)
mice is associated with impaired macrophage phagocytosis of
In the present study, we further investigated the effects of the 5-LOX metabolite, leukotriene (LT)B
on phagocytosis of
Bone marrow-derived macrophages (BMDMs) from 5-LOX
mice were defective in the uptake and killing of
from the earliest stages of spirochete internalization. BMDMs from mice deficient for the LTB
high-affinity receptor (BLT1
) were also unable to efficiently phagocytose
Addition of exogenous LTB
augmented the phagocytic capability of BMDMs from both 5-LOX
and BLT1
mice, suggesting that the low-affinity LTB
receptor, BLT2, might be involved. Blocking BLT2 activity with the specific antagonist, LY255283, inhibited phagocytosis in LTB
-stimulated BLT1
BMDMs, demonstrating a role for BLT2. However, the lack of a phagocytic defect in BLT2
BMDMs suggested that this was a compensatory effect. In contrast, 12(S)-hydroxyheptadeca-5Z,8E,10E-trienoic acid, a natural BLT2-specific high-affinity ligand, and resolvin E1, a BLT1 agonist, were both unable to boost phagocytosis in BMDMs from either 5-LOX
or BLT1
mice, suggesting a specific role for LTB
in mediating phagocytosis in murine macrophages. This study demonstrates that LTB
promotes macrophage phagocytosis of bacteria via BLT1, and that BLT2 can fulfill this role in the absence of BLT1. |
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ISSN: | 1539-7262 |