Multiplexed and spatiotemporal measurements of glutamate secreted by neurons and bacteria

By applying an in vivo biotinylation platform, glutamate-sensing protein can be easily immobilized on streptavidin-functionalized magnetic microbeads, which expands the detection modality for the spatiotemporal measurements of glutamate secreted by adherent neuronal cells and suspension microbial ce...

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Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2016-09, Vol.52 (79), p.11854-11856
Hauptverfasser:	Lee, S. Y, Kim, E. J, Kim, J. S, Hwang, I, Chung, T. D
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria Bacteria - metabolism Cell Line Corynebacterium glutamicum - metabolism Fluorescence Glutamates Glutamic Acid - metabolism Humans Immobilized Proteins - chemistry Immobilized Proteins - metabolism Microscopy Microscopy, Fluorescence Multiplexing Nanoparticles Neurons Neurons - cytology Neurons - metabolism Neurons - secretion Platforms Signal-To-Noise Ratio Streptavidin - chemistry Streptavidin - metabolism
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Beschreibung
Zusammenfassung:	By applying an in vivo biotinylation platform, glutamate-sensing protein can be easily immobilized on streptavidin-functionalized magnetic microbeads, which expands the detection modality for the spatiotemporal measurements of glutamate secreted by adherent neuronal cells and suspension microbial cells using fluorescence microscopy and microplate photometers. By applying an in vivo biotinylation platform, glutamate-sensing protein can be easily immobilized on streptavidin-functionalized magnetic microbeads, which expands the detection modality for the spatiotemporal measurements of glutamate secreted by adherent neuronal cells and suspension microbial cells using fluorescence microscopy and microplate photometers.
ISSN:	1359-7345 1364-548X
DOI:	10.1039/c6cc04192b