Effect of adenovirus mediated β 2 -AR overexpression on IL-10 level secreted by cardiomyocytes of heart failure rats

The effect of β -adrenergic receptor (AR) overexpression on interleukin (IL)-10 content secreted by cardiomyocytes of heart failure (HF) rats was investigated. A rat model of chronic HF was established by partially banding abdominal aorta and the cardiomyocytes were isolated with collagenase II. The cardiomyocytes were then transfected with adenovirus type 5-ADRβ -enhanced green fluorescent protein (EGFP) for 48 h to observe the changes of β -AR protein expression using western blot analysis. The IL-10 level was detected by ELISA. The experiment was divided into seven groups: Control, HF, HF+EGFP, HF+β , sham, sham+EGFP and sham+β groups. Compared with the sham-operated group, left ventricular diastolic dimension, and left ventricular systolic dimension were increased (P