High-level expression of a recombinant thermostable phytase in Bacillus subtilis

An efficient expression system was developed in Bacillus subtilis for the large scale production of phytase. The phytase gene with a native promoter derived from Bacillus amyloliquefaciens was cloned in the Bacillus expression vector pJH27 under a strong BJ27 promoter and its expression was optimize...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1999, Vol.63 (12), p.2205-2207
Hauptverfasser:	Kim, Y.O. (Korea Research Inst. of Bioscience and Biotechnology, Taejon (Korea R.)), Lee, J.K, Oh, B.C, Oh, T.K
Format:	Artikel
Sprache:	eng
Schlagworte:	BACILLUS SUBTILIS Biological and medical sciences Biotechnology CLONACION MOLECULAR CLONAGE MOLECULAIRE EXPRESION GENICA EXPRESSION DES GENES FITASA FITATOS Fundamental and applied biological sciences. Psychology GENE GENE EXPRESSION GENES Genetic engineering Genetic technics Methods. Procedures. Technologies Modification of gene expression level MOLECULAR CLONING overexpression PHYTASE PHYTATE PHYTATES RECOMBINACION RECOMBINAISON RECOMBINATION
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Beschreibung
Zusammenfassung:	An efficient expression system was developed in Bacillus subtilis for the large scale production of phytase. The phytase gene with a native promoter derived from Bacillus amyloliquefaciens was cloned in the Bacillus expression vector pJH27 under a strong BJ27 promoter and its expression was optimized. The expression of the phytase gene occurred during late exponential growth and the extracellular phytase production was 2.0 units/ml, which constituted over 90% of the total protein. The yield was 100-fold higher than wild type Bacillus amyloliquefaciens DS11
ISSN:	0916-8451 1347-6947
DOI:	10.1271/bbb.63.2205