Comparisons of the transferability of plasmids pCAR1, pB10, R388, and NAH7 among Pseudomonas putida at different cell densities

The transferability of plasmids pCAR1, pB10, R388, and NAH7 was compared using the same donor-recipient system at different cell density combinations in liquid or on a solid surface. pCAR1 was efficiently transferred in liquid, whereas the other plasmids were preferentially transferred on a solid su...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2016-05, Vol.80 (5), p.1020-1023
Hauptverfasser:	Yanagida, Kosuke, Sakuda, Ayako, Suzuki-Minakuchi, Chiho, Shintani, Masaki, Matsui, Kazuhiro, Okada, Kazunori, Nojiri, Hideaki
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Load cell density conjugation Conjugation, Genetic Culture Media - chemistry DNA, Bacterial - genetics DNA, Bacterial - metabolism Gene Expression Profiling Gene Transfer, Horizontal horizontal gene transfer plasmid Plasmids - chemistry Plasmids - metabolism Pseudomonas putida Pseudomonas putida - genetics Pseudomonas putida - metabolism
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Zusammenfassung:	The transferability of plasmids pCAR1, pB10, R388, and NAH7 was compared using the same donor-recipient system at different cell density combinations in liquid or on a solid surface. pCAR1 was efficiently transferred in liquid, whereas the other plasmids were preferentially transferred on a solid surface. Difference of liquid or solid affected the transfer frequency especially at lower cell densities.
ISSN:	0916-8451 1347-6947
DOI:	10.1080/09168451.2015.1127131