The pR plasmid: a tool for studying DNA repair and mutagenesis in prokaryotic and eukaryotic cells

The pR plasmid: a tool for studying DNA repair and mutagenesis in prokaryotic and eukaryotic cells

The pR plasmid, a derivative of R46 plasmid, offers the possibility to have an experimental approach to three important problems related to UV repair and mutagenesis. By using this plasmid we were able to show: a) the pR mucAB genes need the cooperation of uvpl gene product to carry out their UV rep...

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Veröffentlicht in:Annali dell'Istituto superiore di sanità 1989, Vol.25 (1), p.145
Hauptverfasser: Battaglia, P A, Perri, S, Gigliani, F
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cell Line
DNA Repair
Escherichia coli - genetics
Mutation
Plasmids
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Zusammenfassung:The pR plasmid, a derivative of R46 plasmid, offers the possibility to have an experimental approach to three important problems related to UV repair and mutagenesis. By using this plasmid we were able to show: a) the pR mucAB genes need the cooperation of uvpl gene product to carry out their UV repair function; b) the expression of mucAB genes is regulated not only by lexA gene, but by a gene localized in the rep region of pR itself. This gene acts as an antirepressor of lexA; c) mammalian cells show an enhanced resistance to UV light when transformed by pR plasmid carrying the mucAB genes.
ISSN:0021-2571