Developments in spontaneous and coherent Raman scattering microscopic imaging for biomedical applications

First, the potential role of Raman-based techniques in biomedicine is introduced. Second, an overview about the instrumentation for spontaneous and coherent Raman scattering microscopic imaging is given with a focus of recent developments. Third, imaging strategies are summarized including sequential registration with laser scanning microscopes, line imaging and global or wide-field imaging. Finally, examples of biomedical applications are presented in the context of single cells, laser tweezers, tissue sections, biopsies and whole animals. An overview about the instrumentation for spontaneous and coherent Raman scattering microscopic imaging is given with a focus on recent developments.