Bacillus formosensis sp. nov., isolated from pesticide wastewater sample

A Gram-stain-positve, endospore-forming rod (designated as strain CC-LY275T) was isolated from pesticide wastewater sample. The isolate grew at temperature 20-45 ºС, pH 7.0-8.0 and tolerated 6 % (w/v) NaCl. The most closely related strains in terms of 16S rRNA gene sequence similarity were Bacillus horneckiae (97.1 %) and Bacillus oceanisediminis (96.8 %), respectively. The G + C content of the genomic DNA was 37.9 mol%. Strain CC-LY275T was determined to possess iso-C14:0, iso-C15:0 and anteiso-C15:0 as predominant fatty acids. The major polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Cell-wall peptidoglycan contained meso-diaminopimelic acid; menaquinone (MK-7) was the predominant respiratory quinone. According to the distinct phylogenetic, phenotypic and chemotaxonomic properties, the name Bacillus formosensis sp. nov. (type strain CC-LY275T =BCRC 80443T =JCM 18448T) is proposed.