Pr-SNTX, a short-chain three-finger toxin from Papuan pigmy mulga snake, is an antagonist of muscle-type nicotinic acetylcholine receptor (α2βδε)

Three-finger toxins (3FTxs) are one of the major components in snake venoms. In this study, we isolated a cDNA encoding a short-chain 3FTx, Pr-SNTX, from Pseudechis rossignolii. The amino acid sequence of Pr-SNTX is nearly identical to that of its ortholog in Pseudechis australis. Pr-SNTX protein in...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2016, Vol.80 (1), p.158-161
Hauptverfasser:	Yamauchi, Yoko, Kimoto, Hikari, Yang, Xianyu, Filkin, Sergey, Utkin, Yuri, Kubo, Tai, Inagaki, Hidetoshi
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Cloning, Molecular DNA, Complementary - genetics DNA, Complementary - metabolism Elapid Venoms - isolation & purification Elapid Venoms - toxicity Elapidae - physiology Escherichia coli - genetics Escherichia coli - metabolism Gene Expression Gene Library Mice Models, Molecular Molecular Sequence Data Muscle, Skeletal - cytology Muscle, Skeletal - metabolism nAChR Neurons - cytology Neurons - metabolism Neurotoxins - isolation & purification Neurotoxins - toxicity Oocytes - cytology Oocytes - drug effects Oocytes - metabolism Papuan pigmy mulga snake Patch-Clamp Techniques Protein Subunits - antagonists & inhibitors Protein Subunits - chemistry Protein Subunits - genetics Protein Subunits - metabolism Rats Receptors, Nicotinic - chemistry Receptors, Nicotinic - genetics Receptors, Nicotinic - metabolism Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - metabolism Sequence Alignment three-finger toxin venom gland Xenopus laevis
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Beschreibung
Zusammenfassung:	Three-finger toxins (3FTxs) are one of the major components in snake venoms. In this study, we isolated a cDNA encoding a short-chain 3FTx, Pr-SNTX, from Pseudechis rossignolii. The amino acid sequence of Pr-SNTX is nearly identical to that of its ortholog in Pseudechis australis. Pr-SNTX protein inhibited muscle-type (α 2 βδε), but not neuronal α7 nicotinic acetylcholine receptor (nAChR) activity.
ISSN:	0916-8451 1347-6947
DOI:	10.1080/09168451.2015.1065169